STED is a well-known technique to achieve sub-diffraction resolution in fluorescent microscopy. This technique along with two other techniques recieved the noble prize in 2014. On chanllenge in STED is the second intense laser beam to deplete the excited state. One workaround to overcome this to some extend was publish here.

During my PhD I was working on a super-resolution project. I thought I could used this approach to improve my resolution enhancement even further. Althoug it didn’t really workout well for me I thought I leave this code here in case it comes handy for othere. In all honesty, this code was made to replicate the results in the afformentioned article. A similar plugin can be also found for imagej here. Although the imagej plugin (in java) is not as easy as matlab to modify I find it a great starting point for anyone trying a phasor approach. please head to this repository for the matlab code. The file named Phasor_Simulation_1.4.m is file you should be able to run and get the results without hassle. When you run the file you will get three figures; a side by side comparison and 2D phasor plot and a 3D phasor plot. The zip file contains my other codes that resulted in the final version.